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- Title
Simultaneous detection of thiabendazole and carbendazim in foods based on two-color upconversion and magnetic separation nanoparticles fluorescence immunoassay.
- Authors
Wang, Minsi; Zhu, Wenbo; Zhao, Lingyan; Jin, Jingyi; Song, Yang; Liu, Ming
- Abstract
A rapid and sensitive immunoassay for the simultaneous detection of thiabendazole (TBZ) and carbendazim (CBZ) was developed by using magnetic nanoparticles (MNPs) and two-color upconversion nanoparticles (UCNPs). The anti-thiabendazole and anti-carbendazim antibody were linked to NaYF4:Yb, Tm (emission at 474 nm) and NaYF4:Yb, Er (emission at 542 nm) UCNPs as multicolor signal probe, respectively. The thiabendazole and carbendazim coating antigen were connected to magnetic microspheres as capture probe, respectively. Based on a competitive format, capture probes compete with analytes to combine corresponding signal probes. Under excitation at 980 nm, the formed competitive immune-complex fluorescent signal at 474 and 542 nm indicates the concentration of thiabendazole and carbendazim, respectively. Under the optimal conditions, the 50% inhibiting concentration (IC50) and limit of detection (LOD, IC10) were 2.21 and 0.083 ng/mL for thiabendazole and 1.18 and 0.075 ng/mL for carbendazim, respectively. The average recoveries of thiabendazole and carbendazim in foods, including oyster mushroom, shiitake mushrooms, oranges and orange juice, ranged from 83.6 to 100.9% with the coefficient of variance (CV) of 1.0–9.8% for thiabendazole and ranged from 89.6 to 105.9% with the CV of 2.5 to 9.1% for carbendazim. This multiplexed analysis format has potential applications for rapid, efficient detection of multiple food risk factors.
- Subjects
CARBENDAZIM; MAGNETIC separation; MAGNETIC nanoparticles; PHOTON upconversion; IMMUNOASSAY; FLUORESCENCE; PLEUROTUS ostreatus
- Publication
European Food Research & Technology, 2022, Vol 248, Issue 1, p85
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-021-03853-4