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- Title
二苯乙烯苷对H<sub>2</sub>O<sub>2</sub>诱导的MC3T3-E1凋亡的保护作用及机制研究.
- Authors
張金康; 李松林; 魏琳嵐; 鄭子陽; 王斌; 劉東洲; 杜俊杰
- Abstract
Objective The aim of this study was to explore the protective effect and related mechanism of tetrahydroxystilbene glucoside (TSG) on apoptosis of osteoblast precursor cells (MC3 T3-E1) induced by H2O2. Methods MC3 T3-E1 cells pretreated with different concentrations of TSG were treated with 300 μmol/L H2O2 for 24 h. The experiment consisted of 6 groups, including the control group, H2O2, H2O2+TSG (0.1 μmol/L), H2O2+TSG (1 μmol/L), H2O2+TSG (10 μmol/L) and H2O2+NAC (1 mmol/L)(anti-oxidant positive control group). We evaluated the protective effect of TSG on the apoptosis of MC3 T3-E1 cells by MTT assay and Hoechst33258 staining. We tested the production of ROS and MDA by fluorescence microplate reader and MDA assay kit to investigate the oxidative stress status. We tested the production of Bcl-2 and Bax by Western-blotting and the gene expression of Bcl-2 and Bax by RT-PCR. Results Cell death induced by H2O2 alone was observed under microscope, and Hoechst33258 staining showed more cells with nuclear pyknosis and nuclear enrichment, which was improved after being pretreated with different concentrations of TSG. H2O2 alone could lead to a large degree of apoptosis in MC3 T3-E1 cells, and the apoptosis rate decreased after pretreatment with different concentrations of TSG. At the same time, TSG(1-10 μmol/L) could significantly reduce the level of ROS and MDA (P<0.05) and improve the oxidative stress status in MC3 T3-E1 cells. The result of Western-blotting and real-time quantitative RT-PCR demonstrated that pretreatment with TSG could decrease the expression of pro-apoptosis protein Bax and increase the expression of anti-apoptosis protein Bcl-2. Conclusion The apoptosis of MC3 T3-E1 cells increased after induced by H2O2 and decreased by pretreatment with TSG. The mechanism of the protective effect could be through decreasing the expression of pro-apoptosis protein Bax and increasing the expression of anti-apoptosis protein Bcl-2.
- Publication
Chinese Journal of Osteoporosis, 2018, Vol 24, Issue 12, p1596
- ISSN
1006-7108
- Publication type
Article
- DOI
10.3969/j.issn.1006-7108.2018.12.008