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- Title
雷公藤甲素诱导肝细胞选择性自噬的形态学观察.
- Authors
蒋杨雨; 江始源; 曹 丹; 王海燕; 邹昊靖; 李 健
- Abstract
Objective: To observe the level of hepatocyte selective autophagy induced by triptolide. Methods: An autophagy tracing model in hepatocytes was established by rapid tail vein injection of GFP-LC3 plasmids into mice treated with triptolide to observe the hepatocyte autophagy level induced by triptolide in vivo. GFP-LC3 stable expressed L02 cells were transfected with RFP-P62 plasmid, and the selective autophagy profile of L02 and LC3-P62 complex induced by triptolide were dynamically observed by live cell imaging system and fluorescence microscopy. The results of in vivo experiments showed that triptolide significantly induced autophagy in hepatocytes. And a similar trend on the protein expression of LC3II and P62 was demonstrated by Western blot. Results: The results of live cell imaging system and fluorescence microscopy showed the co-localization of LC3 and P62 suggesting that the triptolide could induce autophagy flux. Conclusion: Triptolide induced selective autophagy in hepatocytes and its biological function might be associated with hepatocyte damage repairing after injury.
- Subjects
CELL imaging; IMAGING systems; FLUORESCENCE microscopy; TRIPTOLIDE; PROTEIN expression
- Publication
Progress in Modern Biomedicine, 2023, Vol 23, Issue 3, p206
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2023.02.002