We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Environmental Molecular Detection and Culture for Surveillance of Burkholderia pseudomallei as a Cause of Melioidosis in Perak, Malaysia.
- Authors
Hassan, Abdul Karim Russ; Halim, Aisyah Mardhiah Awang@Abdul; Baharudin, Nur Athirah; Aziz, Anis Amalina Nagur; Yuzzini, Irdina; Sukri, Wan Mohammad Danish Wan Mohd; Ismail, Masitah; Gopalkrishnan, V.
- Abstract
Introduction: Melioidosis is an infectious disease caused by saprophytic soil bacteria, Burkholderia pseudomallei mainly found in tropical region. B. pseudomallei can be transmitted predominantly through contaminated soil and water. The aim of the study were to determine the distribution of B. pseudomallei by molecular detection and culture of B. pseudomallei from selected localities in Perak. Methods: A total of 42 soil samples were taken from the surface and 30cm depth. The soil samples were processed for bacterial DNA extraction for genotypic molecular detection by portable RT-PCR thermocycler by using suitable primers and culture method for phenotypic characteristic. Broth culture media and B. pseudomallei Selective Agar (BPSA) were used by mixing with gentamicin. Forty-two soil samples were taken from three different land use were school field, paddy field and rubber plantation. Nine samples were positive for BPSA culture media and 14 samples were positive for RT-PCR method. Results: The paddy field has the highest detection of B. pseudomallei among all three locations. School field comes in second followed by rubber plantation. Conclusion: Agricultural areas and school fields remains the potential health hazard for melioisosis and precautionary measures need to be address in the prevention of melioidosis.
- Subjects
PERAK (Malaysia); MALAYSIA; BURKHOLDERIA pseudomallei; MELIOIDOSIS; SOIL microbiology; RUBBER plantations; PADDY fields
- Publication
Malaysian Journal of Medicine & Health Sciences, 2023, Vol 19, p2
- ISSN
1675-8544
- Publication type
Article