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- Title
Elevation of lipid peroxide level and production of hydroxy lipids in cultured Hepa-T1 cells by oxidative stressors.
- Authors
Tanaka, Ryusuke; Hatate, Hideo; Ito, Makoto; Nakamura, Takashi
- Abstract
In order to investigate the relationships between levels of lipid peroxides (LPO), hydroxy lipids (L-OH) and of antioxidative enzymes in live fish, cultured cells from tilapia liver (Hepa-T1) were treated with oxidative stressors, 2,2′-azobios(2-amidino-propane) dihydrochloride (AAPH) and H2O2. By treating with 5 mM AAPH, LPO and L-OH of the cells significantly increased time-dependently. In particular, L-OH increased from 7.6 ± 0.7 to 23.6 ± 1.8 nmol/mg protein after 2 h of treatment, and synchronously the glutathione peroxidase (GPx) activity of the cells increased from 259 ± 106 to 1970 ± 135 mU/mg protein. In the case of H2O2, however, no elevation of L-OH was observed. In this case, catalase (CAT) and superoxide dismutase (SOD) activities increased time-dependently. Especially, after 2 h exposure, the CAT activity increased remarkably from 217 ± 21 to 2510 ± 120 mU/mg protein, though the GPx activity did not change significantly. These results suggest that the elevation of the L-OH level is closely associated with the elevation of GPx activity, but not with that of CAT or SOD. However, when vitamin E-enriched Hepa-T1 cells were treated with AAPH and H2O2, the production of LPO and L-OH was suppressed significantly. The findings suggest that vitamin E also plays an important role in the levels of the oxidation products in vivo.
- Subjects
LIPIDS; PEROXIDES; OXIDATIVE stress; ANTIOXIDANTS; FISHES; TILAPIA; CELLS; PEROXIDASE; CATALASE; SUPEROXIDE dismutase; VITAMIN E
- Publication
Fisheries Science, 2006, Vol 72, Issue 3, p665
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2006.01197.x