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- Title
Comparación del cultivo celular de HeLa y HEp-2: Perspectivas de estudios con Chlamydia trachomatis.
- Authors
Carrera Páez, Laura Camila; Pirajan Quintero, Ingrid Daniela; Urrea Suarez, María Camila; Gómez Jiménez, Martha; Monroy Cano, Luz Adriana; Sanchez Mora, Ruth Melida
- Abstract
Objective. The goal of this study was to standardize the cultivation of HeLa cells under different conditions, to be used in Chlamydia trachomatis serovar L2 infection's protocols. Materials and Methods. This study was conducted in four phases that are: 1.Cell Viabilidad by trypan blue technique and his subsequent remark, 2. Standardization HeLa cell culture, 3. Giemsa, 4. Cultivation of Hep-2 cells. Results. As a result of standardization it is determined that the HeLa cell line should be cultured in DMEM, 0.1% L-glutamine, 10% FBS. It was determined that the Giemsa perform better over time of 40 minutes that a clear definition of nucleus and cytoplasm is evident. Comparing against both cell lines HeLa was obtained that the line from day growing well and quickly reaches the expected confluence, the opposed line HEp-2 has a slower growth but achieving the desired confluence the last day.
- Publication
NOVA: Publicación Científica en Ciencias Biomédicas, 2015, Vol 13, Issue 23, p17
- ISSN
1794-2470
- Publication type
Article