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- Title
Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC.
- Authors
Kusumorini, Nindya; Nugroho, Akhmad Kharis; Pramono, Suwijiyo; Martien, Ronny
- Abstract
The majority of active pharmaceutical ingredients in pharmaceuticals are obtained from natural ingredients, one of which is white pepper. White pepper seeds (Piper nigrum L.) contain an alkaloid compound named piperine, which has a broad spectrum of pharmacological effects. This research aimed to isolate the piperine from white pepper seeds to obtain piperine in a more economical, simple, and effective way so that it can be applied to large-scale production in the pharmaceutical industry. Extraction and purification of piperine were carried out using n-hexane and cyclohexane, respectively. The isolated piperine samples were tested for their purity with a set of melting point apparatus, bidimensional TLC, and HPLC. The compound structure was then analyzed using FTIR and NMR spectroscopy. Cyclohexane was successfully used to remove the resinous matter from the nhexane extract of white pepper to produce piperine with 94.57 ± 0.82% of purity. The results of the melting point measurement, FTIR, ¹H-NMR, and 13C-NMR spectra of the isolated piperine were similar to that of the piperine reference substance and agreed with data found in the literature.
- Subjects
HIGH performance liquid chromatography; PEPPERS; MELTING points; BLACK pepper (Plant); NUCLEAR magnetic resonance spectroscopy; FOURIER transform infrared spectroscopy
- Publication
Indonesian Journal of Pharmacy / Majalah Farmasi Indonesia, 2021, Vol 32, Issue 2, p158
- ISSN
0126-1037
- Publication type
Article