We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
INHIBITION EFFECTS OF SOME ANTIMICROBIAL AGENTS FROM SALVIA OFFICINALIS L. ON THE GROWTH OF SELECTED GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIAL STRAINS.
- Authors
Císarová, Miroslava; Ürgeová, Eva; Hleba, Lukáš; Charousová, Ivana; Božik, Matěj; Klouček, Pavel; Maliar, Tibor
- Abstract
In the present study, the antimicrobial activity of sage oil, some of its pure components (1,8-cineole, borneol and α-ß thujone) and sage extract were investigated against selected strains of Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and Gramnegative bacteria (Klebsiella pneumoniae, Escherichia coli). The antimicrobial activities of the used antimicrobials agents were determined by using the micro broth dilution method according to Clinical and Laboratory Standards Institute. The percentage of bacterial growth was determined by measured absorbance on a Microplate Reader Opsys MRTM, Dynex (Chantilly, USA). The best minimum inhibitory concentration (MIC) was found against Gram-positive bacteria S. aureus CCM 4223 by sage essential oil with MIC50 96.05 μg/mL. The results from the percentage growth of tested bacteria showed that antimicrobial agents inhibit more Grampositive, than Gram-negative bacterial strains. The chemical composition of sage essential oil (EO) was analysed using the gas chromatography-mass spectrometry (GC-MS). Sage EO contains primarily α -thujone (14.00 %), borneol (12.90%), camphor (12.90%), (-)-Isopulegol (10.10%), ß-thujone (8.40%) and 1,8- cineole (8.00%). The antioxidant properties of sage oil (71.55%) and sage propylene glycol extract (60.93%) were measured by testing their scavenging effect on DPPH radical activities. Due to antibacterial activity of the tested antimicrobials from sage, the problem of microbial resistance to conventional antibiotics could be treated by pharmaceutical industries to manufacture antibacterial drugs.
- Subjects
ANTI-infective agents; SAGE; GRAM-negative bacteria; BACTERIAL growth; GAS chromatography/Mass spectrometry (GC-MS)
- Publication
Journal of Microbiology, Biotechnology & Food Sciences, 2018, Vol 8, Issue 3, p960
- ISSN
1338-5178
- Publication type
Article
- DOI
10.15414/jmbfs.2018-19.8.3.960-964