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- Title
AGRO-INDUSTRIAL WASTE BASED GROWTH MEDIA OPTIMIZATION FOR BIOSURFACTANT PRODUCTION BY ANEURINIBACILLUS MIGULANUS.
- Authors
Sellami, Mohamed; Khlifi, Achref; Frikha, Fakher; Miled, Nabil; Belbahri, Lassad; Rebah, Faouzi Ben
- Abstract
The present work aimed to optimize a molasses and tuna-processing by-products based new economic medium for biosurfactant (BS) production by a promising strain of Aneurinibacillus migulanus. A culture medium based on a mixture of molasses and supernatants generated from tuna by-products supplemented with oligoelements solution was optimized using the mixture design methodology. Biosurfactant (BS) production and emulsification index (E24) were evaluated. Maximal BS of 2.95 g/l was obtained with a 95:5 (v:v) mixture of molasses and tuna by-product supernatant. However, higher level of E24 (62%) was recorded with medium containing the proportion 5:95 (v:v) of molasses and tuna by-product supernatant. The predicted responses from these mixture proportions were also validated experimentally. Interestingly, oligoelements supplements were not needed to prepare the culture medium. Molasses and tunaby-product, non-conventional substrates, can be used efficiently for BS production by A. migulanus.
- Subjects
AGRICULTURAL wastes; BIOSURFACTANTS; BACTERIAL growth; AGRICULTURAL industries&; the environment; MOLASSES; BIOCHEMICAL substrates; MANAGEMENT
- Publication
Journal of Microbiology, Biotechnology & Food Sciences, 2016, Vol 5, Issue 6, p578
- ISSN
1338-5178
- Publication type
Article
- DOI
10.15414/jmbfs.2016.5.6.578-583