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- Title
The Casuarina NIN gene is transcriptionally activated throughout Frankia root infection as well as in response to bacterial diffusible signals.
- Authors
Clavijo, Fernando; Diedhiou, Issa; Vaissayre, Virginie; Brottier, Laurent; Acolatse, Jennifer; Moukouanga, Daniel; Crabos, Amandine; Auguy, Florence; Franche, Claudine; Gherbi, Hassen; Champion, Antony; Hocher, Valerie; Barker, David; Bogusz, Didier; Tisa, Louis S.; Svistoonoff, Sergio
- Abstract
Root nodule symbioses ( RNS) allow plants to acquire atmospheric nitrogen by establishing an intimate relationship with either rhizobia, the symbionts of legumes or Frankia in the case of actinorhizal plants. In legumes, NIN ( Nodule INception) genes encode key transcription factors involved in nodulation., Here we report the characterization of Cg NIN, a NIN gene from the actinorhizal tree Casuarina glauca using both phylogenetic analysis and transgenic plants expressing either Pro Cg NIN::reporter gene fusions or Cg NIN RNAi constructs., We have found that Cg NIN belongs to the same phylogenetic group as other symbiotic NIN genes and Cg NIN is able to complement a legume nin mutant for the early steps of nodule development. Cg NIN expression is correlated with infection by Frankia, including preinfection stages in developing root hairs, and is induced by culture supernatants. Knockdown mutants were impaired for nodulation and early root hair deformation responses were severely affected. However, no mycorrhizal phenotype was observed and no induction of Cg NIN expression was detected in mycorrhizas., Our results indicate that elements specifically required for nodulation include NIN and possibly related gene networks derived from the nitrate signalling pathways.
- Subjects
RHIZOBIUM; CASUARINA; FILAMENTOUS bacteria; FRANKIACEAE; ACTINORHIZAS
- Publication
New Phytologist, 2015, Vol 208, Issue 3, p887
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.13506