We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of micro RNAs and their mRNA targets during soybean nodule development: functional analysis of the role of miR393j-3p in soybean nodulation.
- Authors
Yan, Zhe; Hossain, Md Shakhawat; Arikit, Siwaret; Valdés‐López, Oswaldo; Zhai, Jixian; Wang, Jun; Libault, Marc; Ji, Tieming; Qiu, Lijuan; Meyers, Blake C.; Stacey, Gary
- Abstract
Plant micro RNAs (mi RNAs) play important regulatory roles in a number of developmental processes. The present work investigated the roles of mi RNAs during nodule development in the crop legume soybean ( Glycine max)., Fifteen soybean small RNA libraries were sequenced from different stages of nodule development, including young nodules, mature nodules and senescent nodules. In order to identify the regulatory targets of the mi RNAs, five parallel analysis of RNA ends ( PARE) libraries were also sequenced from the same stages of nodule development., Sequencing identified 284 mi RNAs, including 178 novel soybean mi RNAs. Analysis of mi RNA abundance identified 139 mi RNAs whose expression was significantly regulated during nodule development, including 12 mi RNAs whose expression changed > 10-fold. Analysis of the PARE libraries identified 533 mi RNA targets, including three nodulation-related genes and eight nodule-specific genes., miR393j-3p was selected for detailed analysis as its expression was significantly regulated during nodule formation, and it targeted a nodulin gene, Early Nodulin 93 ( ENOD93). Strong, ectopic expression of miR393j-3p, as well as RNAi silencing of ENOD93 expression, significantly reduced nodule formation. The data indicate that miR393j-3p regulation of ENOD93 mRNA abundance is a key control point for soybean nodule formation.
- Subjects
PLANT RNA; NUCLEOTIDE sequencing; NUCLEOTIDE sequence; GENOME walking; PLANT development
- Publication
New Phytologist, 2015, Vol 207, Issue 3, p748
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.13365