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- Title
Differential expression of aBombyx moriAHA1 homologue during spermatogenesis.
- Authors
Miyagawa, Y.; Lee, J. M.; Maeda, T.; Koga, K.; Kawaguchi, Y.; Kusakabe, T.
- Abstract
The AHA1 (activator of Hsp90 ATPase) family of proteins were exclusively conserved from yeast to humans, but little is known about their tissue distribution or biological function. In this study, a cDNA for aBombyx moriAHA1 homologue, BmAHA1, was isolated from the testes of larvae on day 3 of the fifth instar using an mRNA differential display method. This cDNA encodes a protein with 341 amino acid residues. Gene expression studies revealed thatBmAHA1mRNA occurred prominently in the testes.In situhybridization and immunostaining showed that theBmAHA1mRNA signals were strongly detected in spermatogonial cells and primary spermatocytes at the fifth larval instar stage, whereas the BmAha1 protein was abundant in round and elongated spermatids at the pupal stage. The localization pattern of the accumulated protein in the elongated spermatids was reminiscent of that reported previously for microtubules, but the BmAha1 protein showed a decrease in apparent concentration during maturation process. The stage- and cell-specific expression indicated that BmAha1 might play a role in silkworm spermatogenesis, especially in postmeiotic differentiation.
- Subjects
SILKWORMS; CATERPILLARS; GENE expression; GENETIC regulation; DEVELOPMENTAL biology; BIOLOGY
- Publication
Insect Molecular Biology, 2005, Vol 14, Issue 3, p245
- ISSN
0962-1075
- Publication type
Article
- DOI
10.1111/j.1365-2583.2005.00553.x