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- Title
The acyl lipid composition of wheat leaves and moss protonemata using a new, non-carcinogenic extraction solvent system.
- Authors
Somersalo, Susanne; Karunen, Pirjo; Aro, Eva-Mari
- Abstract
As chloroform has proved to be carcinogenic we were looking for an alternative solvent system for chloroform:methanol widely used in plant lipid investigations. The lipids from leaves of wheat (Triticum aestivum L. cv. Vakka) and from protonemata of the moss Ceratodon purpureus (Hedw.) Brid. were extracted with two petroleum ether:methanol solvent systems. The polar lipids were separated by two‐dimensional thin‐layer chromatography and the amounts of each lipid class were compared with those obtained from chloroform:methanol (2:1, v/v) extractions. The significantly higher amounts of phosphatidylinositol observed in petroleum ether:methanol (1:1, v/v) extraction suggest that the small amounts reported earlier in plants may be an artefact relating to the solvent system used. As petroleum ether:methanol (1:1, v/v) proved to be at least as good a solvent system as chloroform:methanol (2:1, v/v) we propose it as an alternative extractant for plant polar lipids.
- Subjects
LIPIDS; WHEAT; PROTONEMATA; MOSSES; CARCINOGENICITY testing; EXPERIMENTAL oncology
- Publication
Physiologia Plantarum, 1986, Vol 68, Issue 3, p467
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1986.tb03383.x