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- Title
QUALITY ASSAY OF DISSOLVED AMINO ACIDS AND SUGARS EXCRETED BY CHLAMYDOMONAS REINHARDTH (CHLOROPHYCEAE) AND EUGLENA GRACILIS (EUGLENOPHYCEAE).
- Authors
Vogel, Stephanie L.; Frisch, Harry L.; Gotham, Ivan J.
- Abstract
Dissolved amino acids and sugars produced by Chlamydomonas reinhardtii Dangeard and Euglena gracilis Klebs were assayed using a combination of radiochromatography and membrane separated spinner flasks. Both species produced similar complements of sugars. The sugars produced by the algae included galactose, glucose, maltose and xylase. The amino acid complements produced were different for each species. C. reinhardui excreted aspartiate, leucine, methionine, phenlyalanine, tyrosine and valine. E. gracilis excreted alanine, glutamate, proline and serine. Separation of cells from growth media via membrane filtration produced on overestimate of net mount of dissolved carbon compound excreted by the cells. However, the radiochromatographic spectra for both filtrates and cell-free compartments of the diffusion flask experiments were identical. It is hypothesized that the process of filtration may enhance leakage of labeled cellular pools rather than cellular disruption in the species investigated.
- Subjects
AMINO acids; CARBOHYDRATES; CHLAMYDOMONAS; EUGLENA; RADIOCHROMATOGRAPHY; SUGARS
- Publication
Journal of Phycology, 1978, Vol 14, Issue 4, p403
- ISSN
0022-3646
- Publication type
Article
- DOI
10.1111/j.1529-8817.1978.tb02459.x