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- Title
DNA immunization encoding the secreted nonstructural protein 3 (NS3) of hepatitis C virus and enhancing the Th1 type immune response.
- Authors
Jiao, X.; Wang, R. Y.-H.; Feng, Z.; Hu, G.; Alter, H. J.; Shih, J. W. -K.
- Abstract
To induce a sustained and specific cellular immune response to hepatitis C virus (HCV), DNA immunization of mice was performed using plasmids containing the HCV nonstructural gene 3 (HCV/NS3). Plasmids were constructed such that the NS3 gene was expressed in a secreted form, a nonsecreted form or as a membrane-bound antigen. The plasmid encoding the secreted antigen induced the strongest humoral and cellular immunity and favoured the T-helper type 1 (Th1) pathway as shown by cytokine profiles and switching of antibody subclasses. Our study indicates that DNA immunization with a secreted form of HCV/NS3 is an effective means of inducing primary Th1 immune responses in the murine model.
- Subjects
HEPATITIS C virus; IMMUNIZATION; PLASMIDS; GENE expression; ANTIGENS; CELLULAR immunity
- Publication
Journal of Viral Hepatitis, 2004, Vol 11, Issue 1, p18
- ISSN
1352-0504
- Publication type
Article
- DOI
10.1046/j.1352-0504.2003.00464.x