We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Mode of Action of Cyclic Monoterpenes (−)-Limonene and (+)-α-Pinene on Bacterial Cells.
- Authors
Melkina, Olga E.; Plyuta, Vladimir A.; Khmel, Inessa A.; Zavilgelsky, Gennadii B.
- Abstract
A broad spectrum of volatile organic compounds' (VOCs') biological activities has attracted significant scientific interest, but their mechanisms of action remain little understood. The mechanism of action of two VOCs—the cyclic monoterpenes (−)-limonene and (+)-α-pinene—on bacteria was studied in this work. We used genetically engineered Escherichia coli bioluminescent strains harboring stress-responsive promoters (responsive to oxidative stress, DNA damage, SOS response, protein damage, heatshock, membrane damage) fused to the luxCDABE genes of Photorhabdus luminescens. We showed that (−)-limonene induces the PkatG and PsoxS promoters due to the formation of reactive oxygen species and, as a result, causes damage to DNA (SOSresponse), proteins (heat shock), and membrane (increases its permeability). The experimental data indicate that the action of (−)-limonene at high concentrations and prolonged incubation time makes degrading processes in cells irreversible. The effect of (+)-α-pinene is much weaker: it induces only heat shock in the bacteria. Moreover, we showed for the first time that (−)-limonene completely inhibits the DnaKJE–ClpB bichaperone-dependent refolding of heat-inactivated bacterial luciferase in both E. coli wild type and mutant ΔibpB strains. (+)-α-Pinene partially inhibits refolding only in ΔibpB mutant strain.
- Subjects
MONOTERPENES; BACTERIAL cells; HEAT shock proteins; VOLATILE organic compounds; PHOTORHABDUS luminescens; REACTIVE oxygen species; DNA damage; ABIOTIC stress
- Publication
Biomolecules (2218-273X), 2021, Vol 11, Issue 6, p806
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom11060806