We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lateral Flow Immunoassay Strip Based on Confocal Raman Imaging for Ultrasensitive and Rapid Detection of COVID-19 and Bacterial Biomarkers.
- Authors
Chuan Zhang; Anqi Yang; Sailing He
- Abstract
Rapid and sensitive analysis of proteins in complex biological environments is crucial for the screening and defense against infectious diseases. Here, we show that the lateral °ow immunoassay strip based on confocal Raman imaging can achieve immune analysis at pM and » 104 cfu/mL molecular level for the rapid detection of COVID-19 virus and bacteria. Fluorescent dyes of Alexa 647 were used as Raman markers in the Raman silent region of 1800 cm¡1 and 2800 cm¡1, and colloidal gold nanospheres were used to enhance the Raman signal. Raman imaging was performed with our self-developed confocal Raman microscopy for COVID-19 and Escherichia coli O157: H7 on lateral °ow immunoassay strip. Compared to traditional colloidal gold test strips, the sensitivity of this technology has been significantly improved. This work will promote the widespread application of surface enhanced Raman detection for bacteria and virus, which is of great significance for in vitro screening and disease diagnosis.
- Subjects
IMMUNOASSAY; ESCHERICHIA coli O157:H7; COLLOIDAL gold; SERS spectroscopy; RAMAN microscopy; BIOMARKERS; COVID-19; CHEMILUMINESCENCE immunoassay
- Publication
Progress in Electromagnetics Research M, 2023, Vol 120, p41
- ISSN
1937-8726
- Publication type
Article
- DOI
10.2528/pierm23101104