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- Title
Localized Retroprocessing as aModel of Intron Loss in the Plant Mitochondrial Genome.
- Authors
Cuenca, Argelia; Ross, T. Gregory; Graham, Sean W.; Barrett, Craig F.; Davis, Jerrold I.; Seberg, Ole; Petersen, Gitte
- Abstract
Loss of introns in plant mitochondrial genes is commonly explained by retroprocessing. Under this model, an mRNA is reverse transcribed and integrated back into the genome, simultaneously affecting the contents of introns and edited sites. To evaluate the extent towhich retroprocessing explains intron loss, we analyzed patterns of intron content and predicted RNA editing forwhole mitochondrial genomesof 30 species in themonocot order Alismatales. In this group,wefound an unusually high degree of variation in the intron content, even expanding the hitherto known variation among angiosperms. Some species have lost some two-third of the cis-spliced introns.We found a strong correlation between intron content andediting frequency, and detected27 events inwhich intron loss is consistent with the presence of nucleotides in an edited state, supporting retroprocessing. However, we also detected seven cases of intron loss not readily being explained by retroprocession.Our analyses are also not consistent with the entire length of a fully processed cDNAcopy being integrated intothegenome,but instead indicate that retroprocessingusually occurs for onlypartof the gene. In some cases, several rounds of retroprocessingmay explain intron loss in genes completely devoid of introns.Anumber of taxa retroprocessing seem to be very common and a possibly ongoing process. It affects the entire mitochondrial genome.
- Publication
Genome Biology & Evolution, 2016, Vol 8, Issue 7, p2176
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evw148