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- Title
Levels of HIV-1 in subgingival biofilm of HIV-infected patients.
- Authors
Pavan, Priscila; Pereira, Viviane Tiago; Souza, Rodrigo Carvalho; Souza, Celso Oliveira; Torres, Sandra Regina; Colombo, Ana Paula Vieira; Costa, Luciana Jesus; Sansone, Carmelo; Uzeda, Milton; Gonçalves, Lucio Souza
- Abstract
Aim The aims of the current study were to compare the levels of HIV-1 in the subgingival biofilm ( SHVL) between detectable and undetectable plasmatic HIV-1 viral load ( PHVL) in HIV-infected patients as well as to determine the association of SHVL with PHVL and clinical periodontal parameters. Material and Methods Forty-one HIV-infected individuals were divided into two groups: detectable (21) and undetectable (20) PHVL. Subgingival biofilm samples were obtained for detection and quantification of HIV-1 by real-time RT- PCR. To estimate the effect of co-variables on the outcome undetectable SHVL, the Generalized Estimation Equation ( GEE) was employed. Results Detectable SHVL was observed only in the detectable PHVL group and the detection of the HIV-1 was observed in 40% of these individuals. In the bivariate analysis between co-variables from the individual level and the outcome SHVL, significant difference was observed only for the CD4+ T lymphocytes levels ( p = 0.017). The multiple logistic model demonstrated that only CD4+ T lymphocytes levels had a significant effect on the outcome undetectable SHVL [ OR 8.85 ( CI 3.6-9.2), p = 0.002]. Conclusion HIV-1 can be detected and quantified in the subgingival biofilm of HIV-infected individuals, but these findings are not associated with PHVL and periodontal clinical parameters.
- Subjects
BRAZIL; ACADEMIC medical centers; BIOFILMS; CHRONIC diseases; FISHER exact test; HIV; HIV infections; MULTIVARIATE analysis; PERIODONTITIS; POLYMERASE chain reaction; RESEARCH funding; LOGISTIC regression analysis; VIRAL load; HIGHLY active antiretroviral therapy; REVERSE transcriptase polymerase chain reaction; DATA analysis software; DESCRIPTIVE statistics; MANN Whitney U Test
- Publication
Journal of Clinical Periodontology, 2014, Vol 41, Issue 11, p1061
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/jcpe.12306