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- Title
Number and subtypes of natural killer cells in patients with allergic rhinitis in comparison to healthy subjects.
- Authors
Mesdaghi, Mehrnaz; Vodjgani, Mohammad; Salehi, Eisa; Hadjati, Jamshid; Sarrafnejad, Abdolfattah; Movahedi, Masoud; Berjisian, Farideh; Shahrestani, Tahereh
- Abstract
Background: Allergic rhinitis is a common disorder with great morbidity. Its prevalence has increased during recent years, therefore attracting attentions to its mechanisms. Type 2 cytokines play a major role in allergies. It has been proposed that Natural killer (NK) cells may be able to produce type 2 cytokines. This study was done to evaluate NK cells number and subtypes in patients with allergic rhinitis, comparing healthy subjects. Methods: In a case control study, patients with allergic rhinitis were compared to healthy non-atopic subjects. Allergic rhinitis was diagnosed according to ARIA guidelines. NK cells quantity was studied by staining of peripheral blood mono nuclear cells with anti-CD16-FITC and anti-CD56-PE and evaluated by two color flowcytometry. Intracellular cytokines were evaluated by tri-color flowcytometry. NK cells were separated by magnetic beads, and cultured for 72 hours. Secretion of IL-4, IL-5, IL-10, IL-13, and IFN-γ was measured by ELISA, in stimulated and unstimulated conditions. Results: Patients had more CD16+ CD56+ NK cells than control group. IL-4+ NK cells were significantly higher in patients (p<0.001), but the number of IFN-γ+ NK cells was not different. Cytokine secretion of NK cells was similar in case and control groups. Although IL-13 level after stimulation seemed higher in patients, the difference was not significant. Conclusion: NK cells number is increased in patients with allergic rhinitis and a considerable number of them produce IL-4.
- Subjects
KILLER cells; ALLERGIC rhinitis; CYTOKINES; FLOW cytometry; ENZYME-linked immunosorbent assay
- Publication
Tehran University Medical Journal, 2010, Vol 68, Issue 1, p24
- ISSN
1683-1764
- Publication type
Article