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- Title
Evaluation of human papillomavirus DNA in colorectal cancer and adjacent mucosal tissue samples.
- Authors
Galati, Luisa; Gupta, Purnima; Tufaro, Antonio; Marinaro, Mariarosaria; Saponaro, Concetta; Escobar Marcillo, David Israel; Loisi, Donato; Sen, Rajdip; Robitaille, Alexis; Brancaccio, Rosario N.; Cuenin, Cyrille; McKay-Chopin, Sandrine; Paradiso, Angelo Virgilio; Liška, Václav; Souček, Pavel; Zito, Francesco Alfredo; Hughes, David J.; Tommasino, Massimo; Gheit, Tarik
- Abstract
Background: Although the role of viral agents, such as human papillomavirus (e.g. HPV16, HPV18) in colorectal cancer (CRC) has been previously investigated, results remain inconclusive. Methods: To further evaluate the involvement of oncogenic HPV types in CRC, 40 frozen neoplastic and 40 adjacent colonic tissues collected from Italian patients were analyzed by Luminex-based assays that detect a broad spectrum of HPV types, i.e. Alpha (n = 21), Beta (n = 46) and Gamma HPVs (n = 52). In addition, 125 frozen CRC samples and 70 surrounding mucosal tissues were collected from Czech patients and analyzed by broad spectrum PCR protocols: (i) FAP59/64, (ii) FAPM1 and (iii) CUT combined with Next Generation Sequencing (NGS). Results: Using Luminex-basedassays, DNA from HPV16 was detected in 5% (2/40) CRC tissues from Italian patients. One HPV16 DNA-positive CRC case was subsequently confirmed positive for E6*I mRNA. Cutaneous beta HPV types were detected in 10% (4/40) adjacent tissues only, namely HPV111 (n = 3) and HPV120 (n = 1), while gamma HPV168 (n = 1) and HPV199 (n = 1) types were detected in adjacent and in tumor tissues, respectively. The NGS analysis of the CRC Czech samples identified HPV sequences from mucosal alpha-3 (HPV89), alpha-7 (HPV18, 39, 68 and 70) and alpha-10 species (HPV11), as well as cutaneous beta-1 (HPV20, 24, 93, 98, 105,124) beta-2 (HPV23), beta-3 (HPV49) and gamma-1 species (HPV205). Conclusions: Our findings indicate that HPV types belonging to the mucosal alpha, and the 'cutaneous' beta and gamma genera can be detected in the colonic mucosal samples with a low prevalence rate and a low number of HPV reads by Luminex and NGS, respectively. However, additional studies are required to corroborate these findings.
- Subjects
ITALY; TISSUE analysis; PAPILLOMAVIRUSES; DNA; SEQUENCE analysis; EARLY detection of cancer; GENETIC testing; COLORECTAL cancer; CANCER patients; RESEARCH funding
- Publication
Infectious Agents & Cancer, 2023, Vol 18, Issue 1, p1
- ISSN
1750-9378
- Publication type
Article
- DOI
10.1186/s13027-023-00552-5