We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Arabidopsis thaliana mitochondrial EF-G1 functions in two different translation steps.
- Authors
Suematsu, Takuma; Watanabe, Osamu; Kita, Kiyoshi; Yokobori, Shin-ichi; Watanabe, Yoh-ichi
- Abstract
Translation elongation factor G (EF-G) in bacteria catalyses the translocation of transfer RNA on ribosomes in the elongation step as well as dissociation of post-termination state ribosomes into two subunits in the recycling step. In contrast, the dual functions of EF-G are exclusively divided into two different paralogues in human mitochondria, named EF-G1mt for translocation and EF-G2mt for ribosomal dissociation. Many of the two eukaryotic EF-G paralogues are phylogenetically associated with EF-G1mt and EF-G2mt groups. However, plant paralogues are associated with EF-G1mt and plastid EF-G, not with EF-G2mt. In this study, we phylogenetically and biochemically characterized Arabidopsis thaliana EF-G1mt (AtEF-G1mt) to clarify the factor responsible for the dissociation of ribosomes in plant mitochondria. We showed that eukaryotic EF-G1mts form one monophyletic group separated from bacterial EF-G and are classified into five sister groups. AtEF-G1mt is classified into a different group from its human counterpart. We also demonstrated that AtEF-G1mt catalyses both translocation and ribosomal dissociation, unlike in humans. Meanwhile, AtEF-G1mt is resistant to fusidic acid, an inhibitor of bacterial EF-G. Here, we propose that the functional division is not necessarily conserved among mitochondriate eukaryotes and also that EF-G1mt in organisms lacking EF-G2mt functions in two steps, similar to conventional bacterial EF-G.
- Subjects
ARABIDOPSIS thaliana; PLANT mitochondria; RIBOSOMES; GENETIC translation; CHROMOSOMAL translocation; RIBOSOME recycling factor; TRANSFER RNA; ELONGATION factors (Biochemistry)
- Publication
Journal of Biochemistry, 2014, Vol 155, Issue 2, p107
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvt105