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- Title
A nuclear protein tyrosine phosphatase activates p53 and induces caspase-1-dependent apoptosis
- Authors
Gupta, Sanjeev; Radha, Vegesna; Sudhakar, Ch.; Swarup, Ghanshyam
- Abstract
PTP-S2/TC45 is a nuclear protein tyrosine phosphatase, which induces p53-dependent apoptosis. Here we show that the p53 protein level increased in MCF-7 cells in response to PTP-S2 overexpression. PTP-S2-induced p53 protein was transcriptionally active and it could activate caspase-1 gene expression from endogenous as well as ectopic promoter. Coexpression of an active site mutant of procaspase-1 strongly inhibited PTP-S2-induced apoptosis. Mutant procaspase-1 also inhibited apoptosis induced by p53 overexpression or doxorubicin treatment, which induce caspase-1 gene expression. In contrast, apoptosis induced by staurosporine or cycloheximide, which do not increase caspase-1 gene expression, was not affected by mutant procaspase-1. These results suggest that caspase-1 may be one of the mediators of p53-dependent apoptosis in human cells.
- Subjects
PROTEIN-tyrosine phosphatase; APOPTOSIS
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p61
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03628-1