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- Title
Evidence for two interaction regions for phosphatidylinositol(4,5)-bisphosphate on mammalian profilin I
- Authors
Skare, Petra; Karlsson, Roger
- Abstract
The binding of phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P2) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross-linked profilin:β-actin. The result is in agreement with observations made with several mutant profilins and provides strong evidence for two regions on mammalian profilin mediating electrostatic interaction with phosphatidylinositol lipids; one close to the binding site for poly(L-proline), and one partially overlapping with the actin-binding surface. Congruent with this, two plant profilins, which have a reduced number of positive amino acids in one of these regions, displayed a dramatically lower binding to PI(4,5)P2 compared to human profilin I.
- Subjects
PHOSPHOINOSITIDES; ACTIN; LIPIDS
- Publication
FEBS Letters, 2002, Vol 522, Issue 1-3, p119
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02913-7