We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antimicrobial lectin from Schinus terebinthifolius leaf.
- Authors
Gomes, F.S.; Procópio, T.F.; Napoleão, T.H.; Coelho, L.C.B.B.; Paiva, P.M.G.
- Abstract
Aims Schinus terebinthifolius leaves are used for treating human diseases caused by micro-organisms. This work reports the isolation, characterization and antimicrobial activity of S. terebinthifolius leaf lectin ( Ste LL). Methods and Results The isolation procedure involved protein extraction with 0·15 mol l−1 NaCl, filtration through activated charcoal and chromatography of the filtrate on a chitin column. Ste LL is a 14- kDa glycopeptide with haemagglutinating activity that is inhibited by N-acetyl-glucosamine, not affected by ions ( Ca2+ and Mg2+) and stable upon heating (30-100°C) as well as over the pH 5·0-8·0. The antimicrobial effect of Ste LL was evaluated by determining the minimal inhibitory ( MIC), bactericide ( MBC) and fungicide ( MFC) concentrations. Lectin was active against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis and Staphylococcus aureus. Highest bacteriostatic and bactericide effects were detected for Salm. enteritidis ( MIC: 0·45 μg ml−1) and Staph. aureus ( MBC: 7·18 μg ml−1), respectively. Ste LL impaired the growth ( MIC: 6·5 μg ml−1) and survival ( MFC: 26 μg ml−1) of Candida albicans. Conclusions Ste LL, a chitin-binding lectin, purified in milligram quantities, showed antimicrobial activity against medically important bacteria and fungi. Significance and Impact of the Study Ste LL can be considered as a new biomaterial for potential antimicrobial applications.
- Subjects
LECTINS; BRAZILIAN pepper tree; ANTIFUNGAL agents; FILTERS &; filtration; HYDROGEN-ion concentration; N-acetylglucosamine; ESCHERICHIA coli
- Publication
Journal of Applied Microbiology, 2013, Vol 114, Issue 3, p672
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/jam.12086