We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cutaneous Low-Dose Radiation Increases Tissue Vascularity through Upregulation of Angiogenic and Vasculogenic Pathways.
- Authors
Thanik, Vishal D.; Chang, Christopher C.; Lerman, Oren Z.; Greives, Matthew R.; Le, Huong; Warren, Stephen M.; Schneider, Robert J.; Formenti, Sylvia C.; Saadeh, Pierre B.; Levine, Jamie P.
- Abstract
Background/Aims: Neovascularization involves angiogenesis and vasculogenesis mediated by cytokines and soluble chemokines. The predominant stimulus is ischemia, however, recent data suggest that ionizing radiation (IR) has angiogenic potential. In this study we evaluated whether IR increases vascularity and perfusion in vivo. Methods: In wild-type mice, a full-thickness, pedicled skin flap was created and isolated for localized irradiation at a dose of 5 Gy. Serial Doppler analysis of the flap was performed. The skin flaps were then harvested at various time points for vascularity and histologic analysis. Blood was concurrently harvested for serum and hematopoietic progenitor cell population analysis. Results: IR to an ischemic flap augmented the angiogenic cytokines SDF-1 and VEGF. Serum MMP-9 and s-kit levels, which are critical for progenitor cell mobilization, were also increased. When hematopoietic progenitor cells were evaluated by Sca1+/Flk1+ cells, a correlate 2-fold increase was seen compared to controls. When the flaps were examined, both vascularity and perfusion were increased. Conclusion: In this study we demonstrate that local, low-dose IR upregulates angiogenic chemokines and results in progenitor cell mobilization to the systemic circulation. There is a resultant increase in the vascularity of the irradiated flap, suggesting that the pro-angiogenic effects of IR can be harnessed locally. Copyright © 2010 S. Karger AG, Basel
- Subjects
NEOVASCULARIZATION; IONIZING radiation dosage; CYTOKINES; SERUM; PERFUSION; LABORATORY mice
- Publication
Journal of Vascular Research, 2010, Vol 47, Issue 6, p472
- ISSN
1018-1172
- Publication type
Article
- DOI
10.1159/000313875