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- Title
Consumption of Dietary Cod Protein Improves Insulin Sensitivity by Augmenting Skeletal Muscle PI 3-Kinase Activity in Overweight Insulin-Resistant Human Subjects.
- Authors
Ouellet, Veronique; Marcotte, Bruno; Marois, Julie; Weisnagel, Stanley J.; Marette, Andre; Jacques, Helene
- Abstract
We have previously reported that feeding cod protein to high-fat fed rats prevents the development of skeletal muscle insulin resistance and normalizes insulin-stimulated phosphatidylinositol 3-kinase (PI 3-kinase) activity, leading to an improved translocation of GLUT4 to muscle T-tubules. Thus, the aim of the present study was to compare the effects of cod protein to those of other sources of animal proteins on insulin sensitivity and muscle PI 3-kinase activity in insulin-resistant human subjects. Overweight (BMI>27kg/m[sup 2]) insulin-resistant men and women we(e fed a cod protein diet (CP) and a similar diet containing lean beef, pork, veal, eggs, milk and milk products (BPVEM) for 4 wk in a crossover design study. Both experimental diets were formulated to differ only in protein source thus providing equivalent amounts of dietary fibres, monounsaturated, polyunsaturated and saturated fatty acids (1.1:1.7:1.0) as well as n-3 polyunsaturated fatty acids. Hyperinsulinemic-euglycemic clamp studies revealed that feeding dietary cod protein increased insulin sensitivity by 29% whereas BPVEM decreased it by 3%, showing a significant improvement in insulin sensitivity in subjects consuming CP compared with BPVEM (P=0.03). In order to elucidate the mechanism by which dietary cod protein improves insulin sensitivity in these subjects, vastus lateralis muscle biopsies were taken before and after insulin infusion and IRS-l-associated PI 3-kinase activity was determined. We found that in the BPVEM diet, insulin only caused a small (1.27±0.12 fold over basal, NS) and non-significant activation of IRS-1 -associated PI 3-kinase whereas in the CP diet, insulin significantly activated IRS-l-associated PI 3-kinase (1.55±0.18 fold over basal, P=0.001). In conclusion, our results suggest that dietary cod protein improves insulin sensitivity in insulin-resistant subjects by augmenting insulin action on skeletal muscle PI 3-kinase activity.
- Subjects
PROTEINS; CODFISH; INSULIN resistance; OBESITY; FATTY acids; DIETARY fiber
- Publication
Diabetes, 2007, Vol 56, pA447
- ISSN
0012-1797
- Publication type
Article