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- Title
Effects of CS, a BuOH-Soluble Fraction of Cassiae Semen Methanolic Extract, on COX-2 Expression in Renal Cortex of STZ-Induced Diabetic Rats and Cultured Glomerular Mesangial Cells.
- Authors
Dong Ho Jung; Young Sook Kim; Eun Jin Sohn; Chan-Sik Kim; Yun Mi Lee; Jeong Lim Yoo; Ga Young Lee; Jin Sook Kim
- Abstract
Cassiae semen is widely used for antipyrogenic, to improve eyesight, and to relax bowels in Korean traditional herbal medicines and prescriptions. Previous study showed that a BuOH-soluble fraction of Cassiae semen methanolic extract (CS) inhibits in-vitro formation of advanced glycation end products (AGEs) and also preventive effect on the development of diabetic nephropathy in STZ-induced diabetic rats. Using HPLC analysis of CS, three compounds are identified as rubrofusarin-6-O-β-D-gentiobioside, toralactone-9-O-β-D-gentiobioside, and cassiaside. Cyclooxygenase (COX)-2 is a potent proinflammatory molecule, plays an important role in inflammatory disease including diabetes and its expression was up regulated in experimental models of diabetic nephropathy. Aim of this study was to examine anti-inflammatory effect of CS by analyzing COX-2 expression in renal cortex of STZ-induced diabetic rats and AGEs treated glomerular mesangial cells (MC). Reverse transcription-polymerase chain reactions and enzyme-linked immunosorbent assay were used to evaluate COX-2 mRNA and its enzyme product, respectively. We observed increased COX-2 mRNA expression in cortical tissues from STZ-diabetic rats relative to corresponding control rats. Treating rat MC with AGEs also significantly increased COX-2 mRNA expression compare to corresponding untreated MC. However, administration of CS (200 mg/kg) to STZ-diabetic rats (6 weeks old) for 12 weeks significantly reduced COX-2 mRNA expression by 2 fold in cortical tissues (2.26±0.11 vs. 1.08±0.05, p<0.01) than the STZ-diabetic rats. In our in-vitro experiment, CS treatment of rat MC inhibits AGE induced COX-2 mRNA expression by 2 fold (1.89±0.09 vs. 0.98±0.06, p<0.05) that the untreated control. Thus it is worthwhile to be investigated further for its potential pharmacological effects in diabetic nephropathy.
- Subjects
CASSIA siamea; THERAPEUTIC use of plant extracts; KIDNEY cortex; ANTI-inflammatory agents; DIABETIC nephropathies; LABORATORY rats; PREVENTION
- Publication
Diabetes, 2007, Vol 56, pA155
- ISSN
0012-1797
- Publication type
Article