We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Extracellular Vesicles Released from Cancer Cells Promote Tumorigenesis by Inducing Epithelial to Mesenchymal Transition via β-Catenin Signaling.
- Authors
Malyla, Vamshikrishna; Paudel, Keshav Raj; Rubis, Gabriele De; Hansbro, Nicole G.; Hansbro, Philip M.; Dua, Kamal
- Abstract
Lung cancer is the leading cause of cancer-related deaths globally, in part due to a lack of early diagnostic tools and effective pharmacological interventions. Extracellular vesicles (EVs) are lipid-based membrane-bound particles released from all living cells in both physiological and pathological states. To understand the effects of lung-cancer-derived EVs on healthy cells, we isolated and characterized EVs derived from A549 lung adenocarcinoma cells and transferred them to healthy human bronchial epithelial cells (16HBe14o). We found that A549-derived EVs carry oncogenic proteins involved in the pathway of epithelial to mesenchymal transition (EMT) that are regulated by β-catenin. The exposure of 16HBe14o cells to A549-derived EVs resulted in a significant increase in cell proliferation, migration, and invasion via upregulating EMT markers such as E-Cadherin, Snail, and Vimentin and cell adhesion molecules such as CEACAM-5, ICAM-1, and VCAM-1, with concomitant downregulation of EpCAM. Our study suggests a role for cancer-cell-derived EVs to induce tumorigenesis in adjacent healthy cells by promoting EMT via β-catenin signaling.
- Subjects
EPITHELIAL-mesenchymal transition; EXTRACELLULAR vesicles; CELL adhesion molecules; CANCER cells; ONCOGENIC proteins; CATENINS; WNT proteins; WNT signal transduction
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 4, p3500
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24043500