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- Title
Thermodynamic Swings: How Ideal Complex of Cas9–RNA/DNA Forms.
- Authors
Zhdanova, Polina V.; Lomzov, Alexander A.; Prokhorova, Daria V.; Stepanov, Grigory A.; Chernonosov, Alexander A.; Koval, Vladimir V.
- Abstract
Most processes of the recognition and formation of specific complexes in living systems begin with collisions in solutions or quasi-solutions. Then, the thermodynamic regulation of complex formation and fine tuning of complexes come into play. Precise regulation is very important in all cellular processes, including genome editing using the CRISPR–Cas9 tool. The Cas9 endonuclease is an essential component of the CRISPR–Cas-based genome editing systems. The attainment of high-specificity and -efficiency Cas9 during targeted DNA cleavage is the main problem that limits the practical application of the CRISPR–Cas9 system. In this study, we analyzed the thermodynamics of interaction of a complex's components of Cas9–RNA/DNA through experimental and computer simulation methods. We found that there is a small energetic preference during Cas9–RNA/DNA formation from the Cas9–RNA and DNA/DNA duplex. The small difference in binding energy is relevant for biological interactions and could be part of the sequence-specific recognition of double-stranded DNA by the CRISPR–Cas9 system.
- Subjects
CRISPRS; ENDONUCLEASES; GENOME editing; BINDING energy; MOLECULAR dynamics; THERMODYNAMICS; DNA
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 16, p8891
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23168891