We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Neuroinflammation and Neuronal Loss in the Hippocampus Are Associated with Immediate Posttraumatic Seizures and Corticosterone Elevation in Rats.
- Authors
Komoltsev, Ilia G.; Frankevich, Stepan O.; Shirobokova, Natalia I.; Volkova, Aleksandra A.; Onufriev, Mikhail V.; Moiseeva, Julia V.; Novikova, Margarita R.; Gulyaeva, Natalia V.
- Abstract
Hippocampal damage after traumatic brain injury (TBI) is associated with late posttraumatic conditions, such as depression, cognitive decline and epilepsy. Mechanisms of selective hippocampal damage after TBI are not well understood. In this study, using rat TBI model (lateral fluid percussion cortical injury), we assessed potential association of immediate posttraumatic seizures and changes in corticosterone (CS) levels with neuroinflammation and neuronal cell loss in the hippocampus. Indices of distant hippocampal damage (neurodegeneration and neuroinflammation) were assessed using histological analysis (Nissl staining, Iba-1 immunohistochemical staining) and ELISA (IL-1β and CS) 1, 3, 7 and 14 days after TBI or sham operation in male Wistar rats (n = 146). IL-1β was elevated only in the ipsilateral hippocampus on day 1 after trauma. CS peak was detected on day 3 in blood, the ipsilateral and contralateral hippocampus. Neuronal cell loss in the hippocampus was demonstrated bilaterally; in the ipsilateral hippocampus it started earlier than in the contralateral. Microglial activation was evident in the hippocampus bilaterally on day 7 after TBI. The duration of immediate seizures correlated with CS elevation, levels of IL-1β and neuronal loss in the hippocampus. The data suggest potential association of immediate post-traumatic seizures with CS-dependent neuroinflammation-mediated distant hippocampal damage.
- Subjects
NEUROINFLAMMATION; BRAIN injuries; LABORATORY rats; CORTICOSTERONE; HIPPOCAMPUS (Brain); HISTOCHEMISTRY; NEUROGLIA
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 11, p5883
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22115883