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- Title
Intracellular Ca 2+ -Mediated AE2 Is Involved in the Vectorial Movement of HaCaT Keratinocyte.
- Authors
Hwang, Soyoung; Shin, Dong Min; Hong, Jeong Hee
- Abstract
Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.
- Subjects
BICARBONATE ions; PROTEIN expression; WOUND healing; KERATINOCYTES; SKIN injuries; HISTAMINE; CHELATING agents
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 22, p8429
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21228429