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- Title
The Role of Prep1 in the Regulation of Mesenchymal Stromal Cells.
- Authors
Maroni, Giorgia; Panetta, Daniele; Luongo, Raffaele; Krishnan, Indira; La Rosa, Federica; Campani, Daniela; Salvadori, Piero; Iozzo, Patricia; Blasi, Francesco; Penkov, Dmitry; Levantini, Elena; Magli, Maria Cristina
- Abstract
Molecular mechanisms governing cell fate decision events in bone marrow mesenchymal stromal cells (MSC) are still poorly understood. Herein, we investigated the homeobox gene Prep1 as a candidate regulatory molecule, by adopting Prep1 hypomorphic mice as a model to investigate the effects of Prep1 downregulation, using in vitro and in vivo assays, including the innovative single cell RNA sequencing technology. Taken together, our findings indicate that low levels of Prep1 are associated to enhanced adipogenesis and a concomitant reduced osteogenesis in the bone marrow, suggesting Prep1 as a potential regulator of the adipo-osteogenic differentiation of mesenchymal stromal cells. Furthermore, our data suggest that in vivo decreased Prep1 gene dosage favors a pro-adipogenic phenotype and induces a "browning" effect in all fat tissues.
- Subjects
ADIPOGENESIS; STROMAL cells; MESENCHYMAL stem cells; HOMEOBOX genes; ADIPOSE tissues; RNA sequencing
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 15, p3639
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20153639