We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Proteomic Analysis Identified DJ-1 as a Cisplatin Resistant Marker in Non-Small Cell Lung Cancer.
- Authors
Hua-Zong Zeng; Yi-Qing Qu; Wen-Jun Zhang; Bing Xiu; An-Mei Deng; Ai-Bin Liang
- Abstract
The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identified, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.
- Subjects
LUNG cancer; CISPLATIN; CANCER patients; ALKYLATING agents; BIOMOLECULES
- Publication
International Journal of Molecular Sciences, 2011, Vol 12, Issue 6, p3489
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms12063489