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- Title
RESULTS OF MUTATION BREEDING ACTIVITY ONCHRYSANTHEMUM × GRANDIFLORUM (RAMAT.) KITAM. IN POLAND.
- Authors
Zalewska, Małgorzata; Miler, Natalia; Tymoszuk, Alicja; Drzewiecka, Barbara; Winiecki, Janusz
- Abstract
Our researches concerning somatic mutagenesis in Chrysanthemum × grandiflorum (Ramat.) Kitam. induced with ionizing radiation have been carried out since 1977. First significant results were obtained in vivo when irradiated leaves detached from flowering chrysanthemums were applied. Induction of mutations in subsequent experiments was conducted in vitro with the use of the adventitious buds technique for regeneration. Explants were leaves, callus, internodes and single nodes. Except for callus, all explants were taken from irradiated microcuttings growing in vitro during the mutagenic treatment. The dose of X- and gamma- rays involved in our experiments ranged from 15 to 25 Gy. For X-rays therapeutic apparatus THX-250 Medicor was used. Gamma radiation was obtained from Co60 cobalt source generated by Theraton 780 C. Regeneration was conducted on MS medium supplemented with 0.6 mg.dm-3 BAP and 2.0 mg.dm-3 IAA. The most spectacular effects were observed when gamma-rays and in vitro regeneration were used. All of mutants were solid, non-mericlinal and non-sectorial chimeras. Our experiments show that over a short period from a single mother cultivar one can obtain numerous attractive mutants, thus creating new cultivar groups.
- Subjects
POLAND; CHRYSANTHEMUMS; PLANT breeding; SOMATIC mutation; IRRADIATION; ANGIOSPERMS
- Publication
Electronic Journal of Polish Agricultural Universities, 2010, Vol 13, Issue 4, p27
- ISSN
1505-0297
- Publication type
Article