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- Title
Activación de células mononucleares humanas de sangre periférica in vitro con Candida albicans (levadura) viva o muerta, y péptidos liberados al medio.
- Authors
Palma Ramos, Alejandro; Castrillón Rivera, Laura Estela; Becerril Parra, Diana Emma; González Pacheco, José Roberto; Zamora Alvarado, Rubén; Padilla Desgarennes, Carmen
- Abstract
Background: The cell wall of C. albicans is mainly composed of polysaccharides manan, glucan and chitin. Manan polysaccharide represents approximately 15.2% to 22.9% of dry weight and slightly more than 40% of the cell wall of fungus polysaccharides. The D- glucan 1-3 and the D-glucan 1-6- are between 47% and 60% of the dry weight of the cell wall. Other components have been reported, such as protein in quantities ranging between 6% and 25%, lipids between 1% and 7% and chitin between 0.6% and 9% of the weight of the cell wall. Species of Candida opsonizaded are ingested by monocytes and macrophages, but Candida organisms not opsonizaded are phagocytosed only by the latter, mainly through binding to mannose receptor. IFN-γ, normally produced by activated T lymphocytes, is one of the main factors that increase activity phagocytic and destruction of C. albicans in human macrophages. Objective: To demonstrate that it occurs stimulation of human mononuclear cells in vitro with Candida albicans alive, dead, or peptides released into the environment, quantifying the IFN-γ and IL-2 secretion. Material and method: The growth of Candida albicans (ATCC 10231) was carried out in the middle of Sauton, for 5 days at 370ºC, centrifugation and broke the biomass of the medium, adjusted to tube the McFarland Nephelometer 600 2 x 106 CFU/mL saline. Divided into 4 fractions, 2 for use as C. albicans live and 2 as C. albicans dead (was treated with moist heat to 249.8ºF (121ºC) at 15 lbs of pressure for 15 minutes). It takes a fraction of each (strain alive and dead strain) for consideration by opsonization with normal human serum, incubating at 37ºC for 1 h. To the supernatant proteins is precipitated with ammonium sulphate saturated solution, bringing it up to 50% saturation, centrifuged and the precipitate was resuspended in 3 mL of water, dialyzed against water, protein concentration was determined by the Lowry method and adjusted antigen at a concentration of 1 mg/mL. Human mononuclear cells are purified by the method of Boyum and adjusted at a concentration of 2 x 106 cel/mL. Results: With the purpose of stimulating human mononuclear cells in vitro, the use of yeast Candida albicans core heat gives good results in both IL-2 as IFN-γ obtained to stimulate these cells with peptides from the microorganism. Conclusion: It is preferable to stimulate the immune system for induction of the presence of IFN-γ with peptides obtained from the microorganism, or Candida albicans dead heat damp.
- Subjects
CANDIDA albicans; PEPTIDES; IN vitro studies; CYTOLOGY; POLYSACCHARIDES; MICROORGANISMS; BIOMASS
- Publication
Dermatología Revista Mexicana, 2013, Vol 57, Issue 5, p319
- ISSN
0185-4038
- Publication type
Article