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- Title
Isolation of an Antifungal Pathogenesis-Related Protein from Naked Oat (Avena nuda) Seeds.
- Authors
Shi, Yawei; Jian, Liu; Han, Deping; Ren, Yemei
- Abstract
A novel antifungal protein was isolated from naked oat (Avena nuda) seeds by acetone fractionation, (NH4)2SO4 precipitation, Q‐Sepharose ion‐exchange chromatography, chitin affinity chromatography, and Sephacryl S‐200 gel filtration chromatography. The antifungal protein exhibited a molecular mass of 23,760, as measured by gel filtration and SDS‐PAGE. Matrix‐assisted laser desorption ionization–time of flight MS analysis indicated that the protein was homologous with a permatin precursor from A. sativa. The protein from naked oats exhibited antifungal activity against Trametes sp. SQ01 (half‐maximal inhibitory concentration [IC50] = 5.68μM), Trichoderma spp. (IC50 = 0.83μM), and Chaetomium sp. R01.
- Subjects
ANTIFUNGAL agents; OAT proteins; TRAMETES (Polyporaceae); MOLECULAR weights; DISEASE resistance of plants
- Publication
Cereal Chemistry, 2015, Vol 92, Issue 1, p44
- ISSN
0009-0352
- Publication type
Article
- DOI
10.1094/CCHEM-12-13-0251-R