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- Title
Effect of temperature on growth, proton extrusion and membrane potential in maize ( Zea mays L.) coleoptile segments.
- Authors
Waldemar Karcz; Zbigniew Burdach
- Abstract
Abstract  The effects of temperature (5â45C) on endogenous growth, growth in the presence of either indoleacetic acid (IAA) or fusicoccin (FC), and proton extrusion in maize coleoptile segments were studied. In addition, membrane potential changes at some temperatures were also determined. It was found that in this model system endogenous growth exhibits a clear maximum at 30C, whereas growth in the presence of IAA and FC shows the maximum value in the range 30â35C and 35â40C, respectively. Simultaneous measurements of growth and external medium pH indicated that FC at stressful temperatures was not only much more active in the stimulation of growth, but was also more effective in acidifying the external medium than IAA. Also the addition of either IAA or FC to the bathing medium at 30 and 40C did not change the kinetic characteristic of membrane potential changes observed for both substances at 25C. However, the increased temperature significantly decreased IAA and FC-induced membrane hyperpolarization. IAA in the incubation medium, at 10C, brought about additional membrane depolarization (apart from the one induced by low temperature). In contrast to IAA, FC at 10C caused gradual repolarization of membrane potential, which correlated with both FC-induced growth and FC-induced proton extrusion. A plausible interpretation for temperature-induced changes in growth of maize coleoptile segments is that, at least in part, these changes were mediated via a PM H+ATPase activity.
- Subjects
CORN growth; PLANT growth promoting substances; INDOLEACETIC acid; ADENOSINE triphosphatase
- Publication
Plant Growth Regulation, 2007, Vol 52, Issue 2, p141
- ISSN
0167-6903
- Publication type
Article
- DOI
10.1007/s10725-007-9184-0