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- Title
p38 and Extracellular Signal-Regulated Kinases Activations have Opposite Effects on Primary-Cultured Rat Cerebellar Granule Neurons Exposed to Sodium Arsenite.
- Authors
Liu, Xiaona; Gao, Yanhui; Yao, Hongju; Zhou, Lingwang; Pei, Junrui; Sun, Liyan; Wang, Jing; Sun, Dianjun
- Abstract
ABSTRACT Arsenic is a widespread environmental toxicant in the world and regarded as both a carcinogen and an anticarcinogen. The present study was designed to evaluate roles of mitogen-activated protein kinases in sodium arsenite-induced effects on primary-cultured rat cerebellar granule neurons (CGNs). Results revealed a decreased viability of the cells exposed to sodium arsenite (from 0 to 50 μM) in a dose-dependent manner. Annexin V-fluorescein isothiocyanate assay showed that apoptosis was obviously induced by arsenite treatment. High phosphorylation expressions of p38 and extracellular signal-regulated kinases (ERK1/2), but not of c-Jun N-terminal kinases were observed due to arsenite treatment by western blotting analysis. Furthermore, SB203580 (an inhibitor of p38) decreased the percentage of apoptotic cells whereas arsenite-stimulated toxicity was enhanced by U0126 (an inhibitor of ERK1/2). Taken together, these data suggest that p38 contributes to arsenite-induced apoptosis of rat CGNs, but ERK1/2 may involve in cell growth and survival.
- Publication
Journal of Biochemical & Molecular Toxicology, 2014, Vol 28, Issue 4, p143
- ISSN
1095-6670
- Publication type
Article
- DOI
10.1002/jbt.21546