We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Decellularization of kidney tissue: comparison of sodium lauryl ether sulfate and sodium dodecyl sulfate for allotransplantation in rat.
- Authors
Keshvari, Mohammad Amin; Afshar, Alireza; Daneshi, Sajad; Khoradmehr, Arezoo; Baghban, Mandana; Muhaddesi, Mahdi; Behrouzi, Pouya; Miri, Mohammad Reza; Azari, Hossein; Nabipour, Iraj; Shirazi, Reza; Mahmudpour, Mehdi; Tamadon, Amin
- Abstract
An automatic decellularization device was developed to perfuse and decellularize male rats' kidneys using both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) and to compare their efficacy in kidney decellularization and post-transplantation angiogenesis. Kidneys were perfused with either 1% SDS solution for 4 h or 1% SLES solution for 6 h. The decellularized scaffolds were stained with hematoxylin and eosin, periodic acid Schiff, Masson's trichrome, and Alcian blue to determine cell removal and glycogen, collagen, and glycosaminoglycan contents, respectively. Moreover, scanning electron microscopy was performed to evaluate the cell removal and preservation of microarchitecture of both SDS and SLES scaffolds. Additionally, DNA quantification assay was applied for all groups in order to measure residual DNA in the scaffolds and normal kidney. In order to demonstrate biocompatibility of the decellularized scaffolds, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were seeded on the scaffolds. In addition, the allotransplantation was performed in back muscle and angiogenesis was evaluated. Complete cell removal in both SLES and SDS groups was observed in scanning electron microscopy and DNA quantification assays. Moreover, the extracellular matrix (ECM) architecture of rat kidney in the SLES group was significantly preserved better than the SDS group. The hUC-MSCs were successfully migrated from the cell culture plate surface into the SDS and SLES decellularized scaffolds. The formation of blood vessels was observed in the kidney in both SLES and SDS decellularized kidneys. The better preservation of ECM than SDS introduces SLES as the solvent of choice for kidney decellularization.
- Subjects
SODIUM dodecyl sulfate; STAINS &; staining (Microscopy); KIDNEYS; CELL preservation; SURFACE plates; BACK muscles
- Publication
Cell & Tissue Research, 2021, Vol 386, Issue 2, p365
- ISSN
0302-766X
- Publication type
Article
- DOI
10.1007/s00441-021-03517-5