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- Title
A low-cost brewery waste as a carbon source in bio-surfactant production.
- Authors
Nazareth, Talita Corrêa; Zanutto, Conrado Planas; Maass, Danielle; Ulson de Souza, Antônio Augusto; de Arruda Guelli Ulson de Souza, Selene Maria
- Abstract
This work aims to produce bio-surfactant using a brewery waste (trub) as a strategy to reduce production costs related to the substrate, as well as to provide an eco-friendly destination for this residue. Trub is obtained during the boiling of the wort, being mainly composed of proteins and reducing sugars. To evaluate important process parameters on bio-surfactant production, a full factorial design (24) was elaborated, having agitation rate and concentrations of trub, yeast extract, and peptone as independent variables. The highest bio-surfactant concentration achieved was 100.76 mg L−1, where FTIR and Maldi-ToF-MS confirmed functional groups characteristic of peptides and isomers of surfactin in the bio-surfactant extract. Trub, agitation and yeast extract showed statistically significant effects on the response variable (surface tension), where an increase in the agitation rate and in the concentration of yeast extract demonstrated a positive impact on the production of bio-surfactant.
- Subjects
BIOSURFACTANTS; YEAST extract; FACTORIAL experiment designs; BREWERIES; SURFACE tension; SURFACTIN
- Publication
Bioprocess & Biosystems Engineering, 2021, Vol 44, Issue 11, p2269
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-021-02602-x