We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Highly exposed Cu active sites as efficient peroxidase mimics for colorimetric analysis.
- Authors
Hengya Wei; Shushu Chu; Fangning Liu; Shengzhen Li; Yizhong Lu
- Abstract
Nanozymes are nanomaterials with intrinsic enzyme-mimic activity, but their large-scale application is generally limited by their low catalytic activity. Herein, we demonstrated that highly exposed Cu active sites on twodimensional (2D) nitrogen-doped carbon (Cux/NC) can serve as efficient peroxidase-like (POD) catalysts with high atomic utilization. Specially, the uniformly distributed Cu active sites could react with H2 O2 to produce singlet oxygen (¹O2) under acidic conditions, which can efficiently oxidizes colorless 3,3 ', 5,5 ' -tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB). Among various Cux/NC nanozymes studied, the Cu0.14 /NC exhibited smaller maximum catalytic velocities (Vmax) and Menten constant (Km) for TMB and H2 O2. Benefiting from the highly active peroxidase-like activity, the Cu0.14 /NC nanozyme could be successfully applied for the hydroquinone (HQ) and ascorbic acid (AA) detection applications through the inhibitory effect of HQ and AA. More interestingly, α-glucosidase (α-Glu) detection sensing platform could be constructed based on HQ as a signal transmitter, with the detection range ranging from 0 to 12 U/L and the minimum detection limit being 0.68 U/L. This work provides not only an idea for the rational design of highly exposed Cu active sites but also fabricate an effective detection sensing platform for HQ, AA, and α-Glu detection.
- Subjects
PEROXIDASE; COLORIMETRIC analysis; CATALYTIC activity; GLUCOSIDASES; VITAMIN C
- Publication
ChemPhysMater, 2024, Vol 3, Issue 2, p204
- ISSN
2097-0323
- Publication type
Article
- DOI
10.1016/j.chphma.2023.12.001