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- Title
Molecular Identification of the Invasive Species, Nysius cymoides (Spinola, 1837) (Hemiptera: Lygaeidae) in Türkiye.
- Authors
Genç, Hanife Yandayan; Saran, Ceren
- Abstract
The false chinch bug, Nysius cymoides (Spinola, 1837) (Hemiptera: Lygaeidae), is an important pest with a wide host range. It was first discovered in Italy but now causing agricultural damage worldwide, including Türkiye. Specimens of N. cymoides were collected from surveys of the sunflower and corn fields in Malkara-Keşan province, in Edirne in July 2020. Because of the population increase of the pest and their damages to sunflowers and corn fields, the pest identification has become an important issue. We investigated molecular characterization and identification of the pest by using the mitochondrial cytochrome oxidase I (COI) barcode region. Adult and late immature stages of the specimens were examined under a stereozoom microscope and morphologically identified as well. The partial COI gene (659 bp) was amplified by using LCO1490/HCO 2198 and PCR products were sequenced directly for molecular characterization. The sequence was registered GenBank database with accession number OL989232. Molecular analyzes were performed using MEGA X software. As a result, the nucleotide frequencies were A = 33.81%, T/U = 35.79%, C = 16.03%, and G = 14.37%. The distancebased species limitation method applied by using ASAP and ABGD, identified 5 species in the partition with the lowest ASAP score (1.5). The barcode gap distance of this partition with the best ASAP score was determined as 1.7%. Also, the threshold distance was determined as 1.62%. The highest nucleotide identity of the studied specimen (OL989232) was detected as 99.8% with the specimen of N. cymoides from France (KJ541649.1) in GenBank. The objective of this study was the first attempt to identify N. cymoides specimens collected in Edirne based on DNA barcode regions.
- Subjects
TURKEY; INTRODUCED species; NYSIUS; GENETIC barcoding; CYTOCHROME oxidase; POLYMERASE chain reaction
- Publication
COMU Journal of Agriculture Faculty / ÇOMÜ Ziraat Fakültesi Dergisi, 2023, Vol 11, Issue 1, p75
- ISSN
2147-8384
- Publication type
Article
- DOI
10.33202/comuagri.1266923