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- Title
PEPTIDE BASED VACCINE DESIGN FOR THERAPEUTIC INTERVENTION AGAINST HTLV-I : A COMPUTATIONAL APPROACH.
- Authors
Bano, Tasneem; Akhtar, Salman; Siddiqui, Mohd Haris; Arif, Jamal M.; Lohani, Mohtashim; Sayeed, Usman; Khan, M. Kalim A.
- Abstract
Human T-cell leukemia virus type 1 (HTLV-1) is a single-stranded diploid RNA genome (9,030-9,040 nucleotides long) belonging to the deltaretrovirus family that causes adult T- cell leukemia (ATL) and HTLV-1-associated myelopathy and tropical spastic paraparesis (HAM/TSP). As there is neither a satisfactory treatment nor a vaccine against this chronic ailment, hence the purpose of the study is to design in silico based peptide vaccine, to analyse the oncogenic protein (tax) of different strains of HTLV-1 and to determine the conserved region which is further subjected to predict all putative epitope that can provide therapeutic intervention against HTLV-1. A total of four protein sequences (tax) retrieved from UniProtKB database were aligned to determine the sequence conservancy and to screen the potential epitopes, netCTLpan server, IEDB analysis resource were used. Further, final epitope set were subjected for immunogenicity assessment and molecular docking analysis. Three T-cell epitopes predicted as a peptide vaccine represents high affinity to MHC class I ("RVIGSALQF", "SLLFGYPVY", "NLHLLFEEY") and high population coverage against the whole world. We also predicted B-cell epitope "TPPITHTTPNIPPSFLQAMR" using ABCPred online server. Further, toxicity assessment of resulted epitope set confers that all three T-cell epitope as well as predicted B-cell epitope is non-toxic and non mutant and would be a relevant representative of a large proportion of the human population. However, the results require validation through in vivo and in vitro study to prove the effectiveness of these predicted epitopes as a peptide vaccine.
- Subjects
PEPTIDES; HTLV; HTLV diseases; ADULT T-cell leukemia; NUCLEOTIDES; VACCINATION
- Publication
Biochemical & Cellular Archives, 2017, Vol 17, Issue 2, p777
- ISSN
0972-5075
- Publication type
Article