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- Title
基于RNA测序技术鉴定和分析绵羊毛囊发育相关的可变剪接事件.
- Authors
袁晓春; 王翊帆; 王雅艳; 孙昊然; 孟科; 李新海
- Abstract
Alternative splicing is an important molecular mechanism that regulates protein diversity and genetic diversity in animals and plants. In order to identify and analyze the variable splicing during hair follicle development during depilation of Dorper sheep, the Illumina HiSeqTM 4000 sequencing platform was used to sequence the transcriptome of the skin tissue samples in the middle of the side of Dorper adult ewes in September 2019 (peak growth period, S1), January 2020 (telogen, wool growth stops, S2), and March 2020 (early growth period, new wool growth causes old wool to fall off, S3). Using the rMATS software to identify the variable splicing events and differential splicing genes in the samples, 128 033 variable splicing events were identified in three group samples, of which the proportion of skipped exons was the highest, accounting for 75.69%, and the proportion of retained introns was the lowest, accounting for 2.03%. A total of 3 448 significantly (P<0.05) different splicing genes were screened in S1 vs S2, S1 vs S3 and S2 vs S3. The functional enrichment analysis of GO (gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) showed that differential splicing genes were significantly enriched in VEGF (vascular endothelial growth factor) signal pathway, MAPK (mitogen-activated protein kinase) signal pathway, PI3K Akt signal pathway, Rap1 (RAS-related protein 1) signal pathway, Hippo signal pathway, neurotrophic factor signal pathway and other pathways closely related to hair follicle development, indicating that differential splicing genes played an important role in hair follicle development during depilation in Dorper sheep, The key genes (SMAD2, FGFR2, BMP4, BCL2, etc.) that may be involved in the hair follicle development of sheep depilation traits were further screened out. By analyzing the AS events in the hair follicle development process of Dorper sheep during depilation, this paper reveals the relevant genetic regulation mechanism in the process of hair follicle development and the periodic cycle of wool, which provides a theoretical basis for the subsequent analysis and excavation of the function of development related genes in the process of hair follicle development and the periodic change of wool growth during sheep depilation.
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 9, p2056
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.20221409