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- Title
泡球蚴感染青藏高原野生田鼠动物模型的建火.
- Authors
王展; 胥瑾; 王海久; 周瀛; 任利; 阳丹才让; 侯立朝; 樊海宁
- Abstract
Objective To analyze; whether Mierotus fuseus in Qinghai - Tibet Plateau can be used as an animal model for Echinococcus infection. Methods Sixty wild voles (Mierotus fuseus) were equally divided into groups A. B. and C to undergo Echinococcus inoculation through subcutaneous injection, direct transhepatic approach, and percutaneous transhepatic approach, respectively. Three months later, the infection rate and mortality rate were determined. After the model animals were sacrificed. HE staining was performed for the infiltration zone; of lesion (2-3 mm near the lesion) , the area adjacent to the lesion and 50% of the lesion, the center of lesion (non - liquefied area) , the liquefied area of lesion, the maigin of lesion ( not including the normal tissue) . and 70% of the lesion, and the pathological structures of hydatid lesions were observed under a microscope. Protoscoleces were extracted from the lesions for in vitro culture. The; chi - square test was used for comparison of categorical data. Results The; success rate of Echinococcus infection in groups A, B. and C were 60% , 80% , and 70% , respectively, with no significant difference between the three groups (X2 =4. 138. P = 0. 126). The mortality rates of groups A, B. and C were 5% , 20% , and 15% , respectively, with no significant difference between the three groups (X2 =5. 201 , P - 0. 107). HE staining showed that the red - stained vesicles had thin, discontinuous cutieular layers, with massive inflammatory cell infiltration around the vesicles; no protoscoleces were observed in all sections. After in vitro culture and staining of the lesion tissue, a large number of live protoscoleces were observed, unstained and with motility. Conclusion This study confirms that Mierotus fuseus, an endemic species in Qinghai - Tibet Plateau, can be used as an animal model for Echinococcus infection. Subcutaneous injection is an appropriate method for Echinococcus inoculation.
- Publication
Journal of Clinical Hepatology / Linchuang Gandanbing Zazhi, 2018, Vol 34, Issue 2, p373
- ISSN
1001-5256
- Publication type
Article
- DOI
10.3969/j.issn.1001-5256.2018.02.031