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- Title
Overexpression, purification, crystallization and preliminary X-ray diffraction of the nisin resistance protein from Streptococcus agalactiae.
- Authors
Khosa, Sakshi; Hoeppner, Astrid; Kleinschrodt, Diana; Smits, Sander H. J.
- Abstract
Nisin is a 34-amino-acid antimicrobial peptide produced by Lactococcus lactis belonging to the class of lantibiotics. Nisin displays a high bactericidal activity against various Gram-positive bacteria, including some human-pathogenic strains. However, there are some nisin-non-producing strains that are naturally resistant owing to the presence of the nsr gene within their genome. The encoded protein, NSR, cleaves off the last six amino acids of nisin, thereby reducing its bactericidal efficacy. An expression and purification protocol has been established for the NSR protein from Streptococcus agalactiae COH1. The protein was successfully crystallized using the vapour-diffusion method in hanging and sitting drops, resulting in crystals that diffracted X-rays to 2.8 and 2.2 Å, respectively.
- Subjects
NISIN; PROTEINS; STREPTOCOCCUS agalactiae; GENETIC overexpression; CRYSTALLIZATION; X-ray diffraction
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2015, Vol 71, Issue 6, p671
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X15006226