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- Title
The culturability of intestinal bacteria of Japanese coastal fish.
- Authors
SUGITA, Haruo; KUROSAKI, Mikihei; OKAMURA, Tosihiro; YAMAMOTO, Sachi; TSUCHIYA, Chiyumi
- Abstract
This article presents a study undertaken to investigate the culturability of intestinal bacteria in Japanese coastal fish, including eight species, for a better understanding of intestinal microbial communities of coastal fish. The following specimens were collected: bambooleaf wrasse Pseudolabrus japonicas; cupid wrasse Thalassoma cupido; scribbled toby Canthigaster rivulata; grass puffer Fugu niphobles; largescale black-fish Girella punctata; smallscale blackfish Girella melanichthys; footballer Microcanthus strigatus; and gray mullet Mugil cephalus. Intestinal contents from four specimens of gray mullet were evenly mixed, fixed with a ninefold volume of four percent paraformaldehyde, and the bacterial community was analyzed by the fluorescent in situ hybridization (FISH) method.
- Subjects
BACTERIAL cultures; GUT microbiome; WRASSES; LABRUS; THALASSOMA; FUGU; STRIPED mullet
- Publication
Fisheries Science, 2005, Vol 71, Issue 4, p956
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2005.01050.x