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- Title
Application value of metagenomic next-generation sequencing in hematological patients with high-risk febrile neutropenia.
- Authors
Xiao Wang; Huiye Zhan; Nan Zhang; Shan Zhang; Yanrong Shuai; Xiaojuan Miao; Yilan Liu; Ling Qiu; Shihui Ren; Sihan Lai; Ying Han; Hao Yao; Xupai Zhang; Fangyi Fan; Haoping Sun; Hai Yi
- Abstract
Background: Metagenomic next-generation sequencing (mNGS) is a novel noninvasive and comprehensive technique for etiological diagnosis of infectious diseases. However, its practical significance has been seldom reported in the context of hematological patients with high-risk febrile neutropenia, a unique patient group characterized by neutropenia and compromised immune responses. Methods: This retrospective study evaluated the results of plasma cfDNA sequencing in 164 hematological patients with high-risk febrile neutropenia. We assessed the diagnostic efficacy and clinical impact of mNGS, comparing it with conventional microbiological tests. Results: mNGS identified 68 different pathogens in 111 patients, whereas conventional methods detected only 17 pathogen types in 36 patients. mNGS exhibited a significantly higher positive detection rate than conventional methods (67.7% vs. 22.0%, P < 0.001). This improvement was consistent across bacterial (30.5% vs. 9.1%), fungal (19.5% vs. 4.3%), and viral (37.2% vs. 9.1%) infections (P < 0.001 for all comparisons). The anti-infective treatment strategies were adjusted for 51.2% (84/164) of the patients based on the mNGS results. Conclusions: mNGS of plasma cfDNA offers substantial promise for the early detection of pathogens and the timely optimization of anti-infective therapies in hematological patients with high-risk febrile neutropenia.
- Subjects
FEBRILE neutropenia; NUCLEOTIDE sequencing; METAGENOMICS; COMMUNICABLE diseases; CELL-free DNA
- Publication
Frontiers in Cellular & Infection Microbiology, 2024, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2024.1366908