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- Title
Best BLAST hit alone cannot be used as evidence of fraud.
- Authors
Díaz-Arce, Natalia; Rodríguez-Ezpeleta, Naiara
- Abstract
Authentication of anglerfish species (Lophius spp.) by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and forensically informative nucleotide sequencing (FINS) methodologies. We thus examined the sequences corresponding to those albacore-labelled tuna products (MW557512, MW557513, MW557514) claimed to be mislabelled bluefin tuna due to a best BLAST hit with sequence EU562888, belonging to I T. thynnus i according to GenBank. Accurate genetic based seafood product traceability requires developing approaches that unequivocally discriminate between species, for which it is essential to understand intra-specific variability as well as each species' evolutionary context. As shown above, questioning our understanding of the evolutionary history of the species under investigation is essential for seafood fraud studies and could avoid errors such as the one made by Blanco-Fernandez et al. when they report mislabeling of albacore products.
- Subjects
FRAUD; BLUEFIN tuna; BIGEYE tuna; YELLOWFIN tuna; MITOCHONDRIAL DNA; IDENTIFICATION of fishes
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-022-26720-y